rnasy tissue microarray kit  (Qiagen)

Journal: Nature Communications

Article Title: RNF20 links the DNA damage response and metabolic rewiring in lung cancer through HIF1α

doi: 10.1038/s41467-025-60223-4

Figure Lengend Snippet: a Histological analysis of representative lung sections of wild-type ( n = 20) and Rnf20 + /- ( n = 32) mice at one year of age; tumors are indicated by arrows. Scale bars, 200 µm. 1, 2 – normal lung tissue; 3, 5 – lesions with adenocarcinoma (AD) characteristics; 4, 6 – lesions with small cell lung carcinoma (SCLC) characteristics. b High magnification images of lesions with AD characteristics (left) and with SCLC characteristics (right). Scale bars, 20 µm. c Immunohistochemical staining of lungs of Rnf20 + /- mice for CGRP, Syp, and CD45 (top panels, 1, 2, 3); TTF1, CD45, and Ki67 (middle panels, 4, 5, 6). Scale bars, 200 µm. The bottom panels show immunostaining for TTF1, Napsin A, and p63 (bottom panels, 7, 8, 9). Scale bars, 100 µm. d Immunohistochemistry of lung tumor tissue samples from a microarray stained with an anti-RNF20 antibody. Scale bars, 100 µm. e Relative staining intensity (H-Score) for RNF20 in lung tumors of different types and grades. n = 70 for AD ( n = 20 Grade 1, n = 30 Grade 2, n = 20 Grade 3); n = 22 for SCLC and n = 20 for normal lung (NL). f, h Normalized expression of RNF20 in normal ( n = 68) and AD ( n = 524) ( f ) or RNF40 ( h ) in normal ( n = 59) and AD ( n = 515) patients in TCGA datasets. FPKM, fragments per kilobase of exon model per million mapped fragments. g, i Overall survival (Kaplan-Meier plot) of lung AD patients expressing high ( n = 336) vs low ( n = 336) levels of RNF20 (probe 222683_at) ( g ) or high ( n = 580) vs low ( n = 581) levels RNF40 (probe 206845_s_at) ( i ). j Immunohistochemistry of lungs from intratracheal (i.t) injection or surgical resection models stained with an anti-RNF20 antibody. Scale bar, 400 µm. k Relative staining intensity (H-Score) of RNF20 in i.t and tumor relapse groups ( n = 5). Statistical analysis between two groups ( f, h, k ) was performed using an unpaired two-tailed Student’s t-test. Multiple comparisons were performed using one-way ANOVA with significances shown relative to NL or between the indicated pairs of AD grades ( e ). Data are shown as mean ± SEM. ‘n’ indicates biological replicates.

Article Snippet: RNA for RNA-Sequencing was isolated by using the RNeasy microarray kit (Qiagen, 73304).

Techniques: Immunohistochemical staining, Staining, Immunostaining, Immunohistochemistry, Microarray, Expressing, Injection, Two Tailed Test

Journal: Nature Communications

Article Title: RNF20 links the DNA damage response and metabolic rewiring in lung cancer through HIF1α

doi: 10.1038/s41467-025-60223-4

Figure Lengend Snippet: a Immunohistochemistry of tissue samples from different types and grades of lung tumors from a tissue microarray stained with HIF1α, ENO1 and LDHA antibodies. Scale bars, 100 µm. b Pearson correlation (r) of the relative staining intensity (H-Score) of RNF20 and HIF1α (top panels), RNF20 and ENO1 (middle panels), and RNF20 and LDHA (bottom panels) in AD and SCLC patients, with a two-sided p-value computed using the t-distribution. n = 70 for AD; n = 22 for SCLC. c Schematic model of the function of RNF20 in lung SCLC and AD development. PNEC pulmonary neuroendocrine cell, AT1 alveolar type 1 cell, AT2 alveolar type 2 cell, RNAPII RNA polymerase II. The figure was created in BioRender .

Article Snippet: RNA for RNA-Sequencing was isolated by using the RNeasy microarray kit (Qiagen, 73304).

Techniques: Immunohistochemistry, Microarray, Staining